田应宽;王霞;周远忠;申旭波;

• 1:遵义医科大学公共卫生学院

摘要(Abstract)：

目的 探讨慢性锰(Mn)暴露对SD大鼠肾脏氧化损伤和核转录相关因子2(Nrf2)信号通路的影响。方法 将48只SPF级健康雄性SD大鼠随机分为高剂量组(50 mg/kg)、低剂量组(10 mg/kg)和对照组(灭菌蒸馏水),每组16只，灌胃染毒12个月。观察肾脏组织病理学变化，检测肾脏中谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量和大鼠血清肌酐(Cre)、尿素氮(BUN)含量，检测核转录相关因子2(Nrf2)、血红素氧化酶-1(HO-1)、醌氧化还原酶1(NQO1)蛋白表达水平。结果 高剂量组大鼠肾脏脏器系数高于低剂量组和对照组(P<0.05);高剂量组肾小球毛细血管扩张，肾小球、肾小管、肾小体内红细胞均增多。与低剂量组和对照组相比，高剂量大鼠血清中Cre、BUN和肾脏MDA水平明显升高，肾脏GSH-Px活性降低(P<0.05)。与对照组相比，低剂量和高剂量组的Nrf2、HO-1、NQO1的蛋白表达均降低(P<0.05)。结论 慢性50 mg/kg Mn染毒暴露可导致肾实质通透性增加，肾脏水肿，肾功异常，其原因可能与Mn所导致的氧化损伤有关；Nrf2信号通路的抑制可能是慢性Mn暴露导致SD大鼠肾脏氧化损伤机制之一。

关键词(KeyWords)： 锰(Mn);肾;氧化损伤;谷胱甘肽过氧化酶(GSH-Px);核转录相关因子2(Nrf2)

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81660552)

作者(Authors): 田应宽;王霞;周远忠;申旭波;

DOI: 10.13421/j.cnki.hjwsxzz.2022.03.003

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