付国庆;石玉琴;朱丽姗;唐啸;芦帷;张玲;常薇;张志兵;

• 1:武汉科技大学医学院公共卫生学院

摘要(Abstract)：

目的研究邻苯二甲酸二乙基己酯(DEHP)对小鼠初级精母细胞(GC-2 spd)氧化应激及凋亡的影响。方法体外传代培养永生化的GC-2 spd细胞,用二甲基亚砜(DMSO)溶解DEHP,以DMSO浓度为0.1%的细胞孔作为对照组,用DEHP终浓度为50、100和200μmol/L的细胞孔作为低、中、高剂量组染毒24 h。分光光度计法测定GC-2 spd细胞丙二醛(MDA)含量,超氧化物歧化酶(SOD)活力和谷胱甘肽过氧化物酶(GSH-Px)活力,流式细胞仪检测不同剂量组细胞凋亡情况。结果与对照组比较,DEHP低、中、高剂量染毒组MDA含量依次升高,差异无明显统计学意义(P>0.05);SOD活力在DEHP高剂量染毒组下降,差异有统计学意义(P<0.05);DEHP低、中剂量染毒组GSH-Px活力明显下降,且差异有统计学意义(P<0.05)。DEHP中、高剂量染毒组细胞凋亡明显高于对照组,且差异有统计学意义(P<0.05)。结论 DEHP可通过诱发小鼠生精细胞氧化应激而诱导细胞凋亡。

关键词(KeyWords)： 邻苯二甲酸二乙基己酯;氧化应激;细胞凋亡;GC-2 spd细胞

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81302401)

作者(Authors): 付国庆;石玉琴;朱丽姗;唐啸;芦帷;张玲;常薇;张志兵;

DOI: 10.13421/j.cnki.hjwsxzz.2015.04.002

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