二氯乙腈对HepG2细胞凋亡的影响Dichloroacetonitrile Effect on HepG2 Cells Apoptosis
翟璐;罗皓;叶美洁;李永崇;黄嘉华;丁梦珂;刘振龙;杨慧;唐焕文;
摘要(Abstract)：
目的探讨饮水消毒副产物二氯乙腈(dichloroacetonitrile,DCAN)对Hep G2细胞凋亡的影响及相关调控机制。方法二甲基亚砜(DMSO)溶解DCAN,以DMSO处理人肝癌细胞(Hep G2细胞)为对照组,分别以50μmol/L、100μmol/L、200μmol/L、400μmol/L DCAN染毒Hep G2细胞为处理组,培养24 h后通过Annexin V/PI流式细胞分析法检测细胞凋亡情况,用荧光测定法观察Caspase 3酶活性,蛋白质印迹法(Western blotting)技术检测Hep G2细胞pro-caspase 3和P53蛋白的表达情况。结果与对照组相比,400μmol/L DCAN处理组可诱导Hep G2细胞凋亡(P<0.05),各处理组细胞内Caspase 3酶活性随着染毒剂量的增加而显著升高(P<0.05)。DCAN作用于Hep G2细胞后pro-Caspase 3和P53蛋白含量呈下降趋势,当染毒浓度达到200μmol/L以上时,pro-Caspase 3和P53蛋白表达明显低于对照组(P<0.05)。结论高浓度的DCAN可通过激活Caspase 3诱导Hep G2细胞凋亡。
关键词(KeyWords)： 二氯乙腈;凋亡;Caspase 3;P53;HepG2细胞
基金项目(Foundation): 广东省大学生创新创业训练计划项目(201510571047);; 湛江市科技攻关计划项目(2015B01006);; 广东医学院面上培育项目(M2014016);广东医学院博士启动项目(2XB13009);广东医学院大学生创新创业训练计划项目(XJ105711425)
作者(Author): 翟璐;罗皓;叶美洁;李永崇;黄嘉华;丁梦珂;刘振龙;杨慧;唐焕文;
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DOI: 10.13421/j.cnki.hjwsxzz.2016.04.001
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